A study published in MicrobiomeJ demonstrates how human organ chip technology can be used to better understand vaginal microbiome-host interactions and evaluate the safety and efficacy of live biotherapeutics products.
consortia engraft and proliferate in the vagina chip, maintain an acid pH, produce both D- and L-lactate, and down-regulate proinflammatory cytokines. Moreover, culture ofalone on-chip increased pH and secretion of inflammatory cytokines, and resulted in epithelial cell injury.
To create the human vagina chip, fibroblasts were seeded first in the basal channel by inverting the chip for 1 h in human fibroblast growth medium. Chips were inverted again, and human vaginal epithelial cells were seeded in the apical channel for 4 h in human vaginal growth medium. The chips were incubated at 37 °C with 5% COovernight under static aerobic conditions.
Customized HBSS medium is composed of 1.26 mM calcium chloride , 0.49 mM magnesium chloride hexahydrate , 0.41 mM magnesium sulfate heptahydrate , 5.33 mM potassium chloride , 0.44 mM potassium phosphate monobasic , 137.93 mM sodium chloride , and 5.56 mM D-glucose . In-house differentiation medium is composed of DMEM , Ham’s F12 , 4 mM L-glutamine , 1 μM hydrocortisone , 1× Insulin-Transferrin-Ethanolamine-Selenium , 20 nM triiodothyronine , 100 μM O-phosphorylethanolamine , 180 μM adenine , 3.2 mM calcium chloride , 2% heat inactivated fetal bovine serum , 1% penicillin-streptomycin , and 4 nM β-Estradiol .
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