New study puts gutmicrobiome at the center of Parkinson's disease pathogenesis NatureComms
Each subject provided one stool sample at one time point; hence each data point represents a unique individual. Each sample was measured and analyzed once. Stool samples were collected at home. Nearly all subjects used the DNA Genotek OMNIgene GUT Collection kit. Five subjects used Fisher Scientific DNA/RNA-free BD BBL Sterile/Media-free Swabs. Collection method was included as a technical covariate in analyses. Upon receipt in the lab, samples were stored in −20 °C freezer.
, with one difference: as our sequencing protocol did not include PCR amplification step, we did not remove duplicated sequences, as these may be actual biological overrepresentation.
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