Study discovers novel molecular mechanism driving chemoresistance and tumor recurrence in gastriccancer hkuniversity NatureComms
Organoids were dissociated from Matrigel using cell recovery solution as aforementioned. Organoids were then processed as per published protocol
. Organoids were stained with the following antibody: ADAR1 , SCD1 , and KHDRBS1 . Organoids were mounted and counterstained with antifade DAPI .Cells were incubated with 5 µg/mL BODIPY 493/503 or 1 µM ER tracker red for 1 h. Cells were fixed with 2% paraformaldehyde, mounted and counterstained with anti-fade DAPI . Slides were imaged by confocal microscopy using Zen 3.0 software and quantified by ImageJ software.
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