Biology behind new drug used to treat triple-negative breast cancer uncovered gumedcenter
. 4175 cells were cultured in DMEM supplemented with 10% Foundation Fetal Bovine Serum . 4T1 cells were cultured in RPMI 1640 supplemented with 10% FBS in a humidified incubator with 5% COat 37 °C. 4T1 cells were stably infected with firefly-expressing lentiviral particles and selected with neomycin, to enable their in vivo tracking in experimental metastasis assays.
Cell cultures were routinely tested for mycoplasma by using MycoAlert sample kit . Additionally, mycoplasma negative status and cell line authentication by STR profiling were performed by IDEXX BioAnalytics.All animal studies were approved by the Institutional Animal Care and Use Committee of the University of Miami or Georgetown University. NSG and BALB/cJ mice were purchased from Jackson Laboratory . Power analysis for all animal experiments was performed using Statmate 2.0 .
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