Researchers assess wastewater sampling for SARS-CoV-2 surveillance UGMYogyakarta PLOSONE SARSCoV2 COVID19 WastewaterSampling
By Bhavana KunkalikarOct 18 2022Reviewed by Aimee Molineux In a recent study published in the PLoS ONE journal, researchers assessed the feasibility of severe acute respiratory syndrome coronavirus 2 surveillance using wastewater sampling.
About the study In the present study, researchers investigated the feasibility of conducting wastewater-based epidemiology surveillance in Indonesia. In three of the five districts in the Special Region of Yogyakarta province, routine WBE surveillance and soil testing were started. As these regions have the maximum coverage of the central wastewater system, the team selected six sub-districts from Yogyakarta city and two each from Sleman district and Bantul district. Furthermore, 12 clustered settlements were chosen that used small community WWTPs. The grab sampling technique or passive sampling was used to collect the samples.
The recovery efficiency and the actual value of SARS-CoV-2 RNA were also reported. Multiple SARS-CoV-2 RNA controls, such as an MS2 phage control at various known concentrations and negative control, were included in each qPCR run. Additionally, the RT-qPCR assay's limit of detection was established.
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