Nanobody treatment shows promise against SARS-CoV-2infection frontiersin NatureComms
T75 flaks of VeroE6 cells were infected with the USA-WA1/2020 , hCoV-19/USA/MD-HP20874/2021 at an MOI of 0.01 for 48 hours. Supernatants were centrifuged at 1500g for 10 minutes and aliquoted and stored at -80°C. Virus titre was determined by TCID50 assay in VeroE6 cells. B.1.1.7 strain was kindly provided by Kristen St. George, Department of health, Wadsworth Center.
using the PichiaPink expression system following the manufacturer’s protocol. Briefly, antibody genes were codon-optimized for expression inα-mating factor pre-sequence secretion signal downstream of theI was used to electroporate stable yeast clones, and expression of the antibody gene was determined bycomplementation on PAD selection plates. For small scale selection of high expressing clones, we utilized a high throughput approach .
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