Researchers find breastcancer protein that could predict chemotherapy sensitivity HMRIAustralia
] . Each of the sublines was maintained in DMEM supplemented with 10% foetal bovine serum , insulin , L-glutamine , and puromycin in humidified 5% COat 37 ˚C. Cells were routinely tested for mycoplasma according to the manufacturer’s recommendations .Cells were seeded into either 96-well plates at 15,000 cells/well and 5000 cells/well , ultra-low attachment 96-well plates at 4000 cells/well , 24-well plates at 1 × 10cells/well .
Cell populations at G1, S, and G2 were normalised to untreated cells within the same phase in order to identify the relative changes.For MCF-7 cells, IncuCyte Annexin-V red reagent was added to the cells at the time of DOX or CIS treatment at a dilution of 1:200 in PBS. Labelled cells were imaged every 3 h using the IncuCyte Zoom live-cell imaging system . Images were analysed using the IncuCyte Zoom 2016B software.
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