Neuroscientists illuminate how braincells deep in the cortex operate in freely moving mice naturemethods
, though we elected to use a plug to minimize the potential for damage to the cortical surface and blood vessels should the cortical surface come into contact with the edges of the region where the bone had been removed.
Freely moving experiments were subsequently conducted from 2 to 10 days after opening the cranial window and positioning the microscope. At the commencement of each recording session, the animal was taken from its home cage, the head gently restrained by holding the handle on the back of the headplate, and the microscope placed onto the intermediate attachment plate. Placement of the microscope was rapid, taking in the order of a few seconds.
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