Discovery of antibody structure could lead to treatment for Crimean Congo Hemorrhagic Fever virus UCRiverside NatureComms
An Octet R8 BLI protein analysis system was used to determine binding kinetics of GP38 mAb. Anti-hIgG Fc Capture sensors from Sartorius which was dipped into baseline 1× Octet kinetics buffer for 60 s. Sensors then encountered a 1 µg/mL antibody solution of either c13G8 or CC5-17 for 150 s as a loading step, following loading, they were then dipped into a second baseline containing 1× Octet Kinetics buffer.
The mixes were incubated for 1 h at +37 C. Then, medium was removed from Vero-E6 cells growing in 96-wells, and quadruplicate wells were infected with 40 µl each at +37 °C, 5% CO2 with periodic shaking. After 1 h, the inocula were removed and the cells overlaid with MEM containing 1.25% carboxymethylcellulose , 4% HI-FBS, sodium pyruvate, and penicillin-streptomycin.
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