The value of whole-exome sequencing as a first-tier genetic test in the diagnosis of skin disorders

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The value of whole-exome sequencing as a first-tier genetic test in the diagnosis of skin disorders
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The genetic etiology by whole-exome sequencing (WES).

By Neha MathurAug 23 2023Reviewed by Lily Ramsey, LLM In a recent article published in BMC Medical Genomics, researchers recruited a cohort of 25 Chinese genodermatoses patients to examine the genetic etiology by whole-exome sequencing .

Researchers have identified over 1,000 monogenic skin disorders, which are highly clinically variable, with clinical symptoms ranging from a generalized phenotype to a predominantly acral blistering phenotype . Overall, there is an unmet need for a method that expedites the discovery of genetic variations to facilitate diagnoses in dermatology, including genodermatoses, which affect different parts of the skin in different ways, i.e., having high clinical heterogeneity.

Furthermore, the researchers used Sanger sequencing to confirm their results and perform prenatal diagnosis. WES accurately diagnosed nine known and nine novel variations in 16 of 25 genodermatoses patients. Of these, 12 variations had a genetic origin, and one even had a de novo origin. Around 48% of the 25 cases had positive family histories, so WES's diagnostic yield was likely higher than the traditional WES diagnostic rate .

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