Scientists explore bat sarbecovirus isolation in Japan CDCgov Sarbecovirus Japan Bats
By Pooja Toshniwal PahariaNov 18 2022Reviewed by Aimee Molineux In a recent study published in Emerging Infectious Diseases, researchers performed bat sarbecovirus isolation experiments using Rhinolophus cornutus bats from several regions in Japan that were phylogenetically situated in the same cluster of viruses related to severe acute respiratory syndrome coronavirus 2 .
About the study In the present study, researchers reported on the identification, isolation, and biological and genetic characterization of sarbecoviruses originating from bats in different Japanese locations. The S RBM of bat isolates from Japan were aligned with those of other sarbecoviruses. For assessing the growth kinetics of sarbecovirus isolates from bats in Japan, R. cornutus bat isolates Rc-os20, Rc-o319, Rc-mk2, and Rc-kw8 or SARS-CoV-2 were inoculated into Vero-RcACE2 [RcACE2, Vero/TMPRSS2 ], Vero-hACE2 , or Vero-ACE2KO cells and the viral titers were determined using plaque assays.
The team observed high similarities among the bat isolates throughout the genome sequence, with the exception of sites coding for the S gene receptor-binding domain and N-terminal domain . However, Rc-kw8 and Rc-o319 NTDs were found to be conserved. No significant recombination was observed among the bat isolates. In the phylogenetic analysis, the isolates were observed in one genetic cluster situated in a genetic clade comprising sarbecoviruses related to SARS-CoV-2.
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