Scientists develop amplicon-based sequencing method for genomic surveillance of human monkeypox virus medrxivpreprint YaleSPH GenomicSurveillance Monkeypox MonkeypoxVirus
By Dr. Sanchari Sinha Dutta, Ph.D.Oct 19 2022Reviewed by Aimee Molineux method has higher viral genome coverage and can even analyze samples with low viral DNA concentration. The study is currently available on the medRxiv* preprint server.
In the current study, scientists have developed and validated a primer scheme for the human monkeypox virus that can be used with amplicon-based sequencing workflows. The findings revealed that both sequencing approaches provide comparable genome coverage for samples with high viral DNA concentrations. However, for samples with low DNA concentrations, amplicon-based sequencing resulted in significantly increased genome coverage.
The findings revealed that the monkeypox primer scheme could be used with various workflows and sequencing platforms to obtain high genome coverage for samples with high or low DNA content. The Ct value obtained from polymerase chain reaction is a surrogate measure of the amount of nucleic acid in samples. The higher the Ct value; the lower the nucleic acid content.
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