Discover why primary cell cultures are necessary for establishing cell lines.
Sponsored Content by Corning Life SciencesNov 16 2022Reviewed by Olivia Frost To establish cell lines, all cell culturing procedures require a primary cell culture. Cells exist in one of two forms when it comes to preparing these cultures: anchorage-dependent or suspension cells. Anchorage-dependent primary cells need to adhere to a solid support to thrive and form adherent cultures, whereas suspension primary cells do not .
There are numerous adherent platforms available, ranging from standard cell culture flasks and multi-layered vessels to fixed-bed bioreactors. And there are microcarriers, a kind of hybrid of adherent and suspension in which adherent cells attached to beads suspended in culture media.
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