Unique genesignature could help predict response to chemotherapy thecrick NatureComms
. The genes were further filtered using the shrunken LFC values prior to GO analysis . Go analysis was carried out using goseq from Bioconductor, accounting for the length bias inherent in RNA-Seq results. Only terms associated with ‘Biological Process’ were considered, and enriched-values were corrected using Benjamini-Hochberg correction . We calculated a sample distance matrix from the VST expression matrix using Euclidian distance.
We plotted the density of distance measures for intra- and inter-tumour distances. We determined the degree of enrichment of RAS84 genes with stable intra-tumour expression, but high inter-tumour variance was assessed relative to the distributions of all expressed genes, in-line with the methods presented in Biswas and colleaguesNo statistical method was used to predetermine sample size. No data were excluded from the analyses.
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