The family of Jagtar Singh Johal, from Dumbarton, claim he was abducted and tortured into making a false confession.
Human rights campaigners have argued there is no basis for the charges, and warned his life is at risk.
"UN legal experts have recognised that his detention is arbitrary and he should immediately be set free. Mr Johal's brother, Gurpreet, told BBC Scotland: "I've said from day one that my brother is innocent, it has taken 1,807 days for Jagtar to know what charges he faces and today he has plead not guilty.
Mr Singh said his brother should be preparing to celebrate his fifth wedding anniversary with his wife on Tuesday.Mr Johal travelled from Scotland to India in October 2017 for his wedding.
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