Classification rate, classification accuracy and taxonomic read classification are heavily impacted by the choice of reference database used, finds a study published in Animal Microbiome.
Species levels. Each bar represents reads classified by Kraken2, using each reference database as shown along the x-axis. The bars represent the percentage of classified reads at various classification status, as shown in the key. “Truth unknown” refers to the reads that originate from genomes that do not have an assigned family or genus. “Unclassified at any level” refers to reads that were not classified to any taxonomic level.
At the species level, use of both of the RefSeq and the Mini databases classified a similar proportion of reads correctly . However, using the RefSeq database incorrectly classified almost the same proportion , whereas using the Mini database incorrectly classified approximately half that amount . As expected for a smaller database, classification with the Mini database had a higher proportion of reads that were unclassified at any level compared to RefSeq .
Table 2 The frequency of families and genera in the Hungate and RUG datasets, and overlap between the two datasetsThe poor performance of RUGs at this level, as demonstrated in classification accuracy for the RUG database, also impacted the RefRUG database. Use of both reference databases including RUGs resulted in over 35% of reads being incorrectly classified.
Danmark Seneste Nyt, Danmark Overskrifter
Similar News:Du kan også læse nyheder, der ligner denne, som vi har indsamlet fra andre nyhedskilder.
Scientists are developing test that could diagnose UTIs in 40 minutes'Over and underdiagnosis of UTIs are both improved by making more accurate diagnosis tests'
Læs mere »
Animal Crossing: New Horizons's DLC Happy Home Paradise on sale for over 25% offAnimal Crossing: New Horizons DLC Happy Home Paradise gets Black Friday price cut:
Læs mere »
Dad at centre of 'hazardous substances' murder probe namedBREAKING: Dad at centre of 'hazardous substances' murder probe named as family pays tribute
Læs mere »
King Charles ban on 'despicable dish' in all royal residencesThe new policy is in place at Buckingham Palace and all other royal residences
Læs mere »
Mystery of grim discovery of body covered in 'potentially hazardous' substancesA huge police scene remains in place on Saturday with murder squad detectives investigating
Læs mere »