Researchers show genetic basis of facial changes in Downsyndrome thecrick
coordinate for each landmark dataset) divided by the number of mesh vertices, was calculated using MorphoJ. Statistical significance of size differences was calculated using a two-tailed unpairedFollowing fixation, embryos were washed in PBS for 3×30 min, then dehydrated through a series of ethanol solutions of 30%, 50%, 70%, 80%, 90% and 100% for 1 h each at room temperature on a nutator and subsequently placed in cassettes.
Sections were washed with PBS, blocked in 20% NGS for 1 h and incubated with goat anti-rabbit IgG Alexa488 and DAPI overnight at 4°C. Finally, sections were washed with PBS and mounted with ProLong Diamond Antifade Mountant .Primary mouse cranial neural crest explant cultures were performed as previously described . Embryos were harvested at E8.5 and immediately placed into ice-cold PBS.
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