In this interview, AZoNetwork speaks with Joe Barco, the Senior Director of Workflow Marketing at Unchained Labs, about the benefits of automated buffer exchange with Big Tuna.
Sponsored Content by Unchained LabsSep 4 2023 Please could you give our readers an overview of the main functions of Big Tuna? Big Tuna enables automated buffer exchange and sample concentration in most biomolecule processing applications. Typically, exchanging buffers from one to another ends up being a bottleneck, requiring many steps and a lot of manual intervention. Big Tuna was designed to automate this process so that people can go and do other things.
For newer gene therapy vector molecules like lipid nanoparticles and AAVs, less work has been done on formulation stability. As those molecules become more frequently assessed for drug development, the understanding of stability is going to become much more critical in those areas. We have a new application on that system where you can reduce a much larger sample volume into a smaller volume. You could run up to about 50 mL per sample, 24 at a time, and concentrate that down to 8 mL of each sample.
We developed Big Tuna with that assumption in mind. We monitor more closely what is happening with each sample. Big Tuna stops the run at different intervals and checks the volume, ensuring that it is aligning with the customer’s desired method. A big component of this is software. People normally do this work manually and do not have to work with an instrument other than perhaps to hit a few buttons. We wanted to make sure that even though this is an automated system, people could very quickly learn what they needed to do to set up their experiment.Can you explain the process used by Big Tuna to remove buffer, and how this proceeds? Big Tuna essentially mimics the process of centrifugation filters except you don’t have to be there.
It figures out how much volume to add back to each sample, determines the percent exchange that has already occurred and estimates the flow rate. The latter number is used to estimate the number of cycles remaining and the amount of time to do the next cycle.
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