CRISPR technology improves Huntington'sdisease symptoms in models ucsandiego NatureNeuro
Integrated DNA Technologies PrimeTime qPCR assay Mm.PT.58.28953379 was used as an internal housekeeping control.Striatal samples were homogenized in a buffer containing 50 mM Tris-HCl , 150 mM NaCl, 0.1% SDS, 1.0% NP-40, 0.5% sodium deoxycholate and 1% protease inhibitors. Then, 30 μg of proteins were separated in a 4–20% gradient gel and transferred to a nitrocellulose membrane. The membrane was blotted with the following primary antibodies: anti-HTT, 1:1,000, catalog no.
High-resolution anatomical images were acquired by using a three-dimensional T2-weighted fast spin echo sequence with the following parameters: echo time /repetition time = 40/700 ms; resolution = 0.1 × 0.1 × 0.25 mm; echo train length = 4; number of average = 2; and flip angle = 40°.
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