Fruitflies help researchers decode genetic link to Alzheimer'sdisease WEHI_research SciReports
Strains were reared on molasses-based Drosophila medium. Briefly, 1.4 kg of molasses is mixed in 3 l of water and 900 g of yeast mixed with 3 l of water before pouring into a 30 l metal pot and placing on a gas burner. The mixture was brought to 100 °C with occasional stirring. 630 g of semolina was mixed with 3 l of water and added. Upon the mixture obtaining a homogeneous consistency, it was cooled to 80 °C prior to the addition of 138 ml Acid Mix and 262 ml Tegosept .
A Zeiss LSM 800 running Zen Blue software was used to visualize and analyse the eye imaginal discs. Serial Z-series were captured in 1 μm sections at 40 × magnification. Fluorophores were imaged using band-pass filters to remove cross-detection between channels and pseudo-coloured for image preparation. Confocal images in generic format were processed using Image J 1.48v, and Adobe Photoshop CS5 Version 12.0.4.I.
Tom40-HA levels were quantified using FIJI . Data points represent different eye discs with the relevant genotype. For each image, 3 μm sections were merged with maximum projection, 5 ommatidia per image were selected based on DAPI staining to identify nuclei, and the average raw Tom40-HA intensity was measured within the selection. For quantification of Mito-YFP area proportion, 5 μm sections were merged and Auto Local Threshold applied to calculate the proportion of total area.
BN-PAGE analysis of solubilised cell membranes from adult fly heads was performed at 4 °C using the Native PAGE Bis–Tris Gel System on 4–16% Bis–Tris gels using NativeMark unstained protein standards. Tagged Tom40 was detected by western blot against the HA peptide.
Danmark Seneste Nyt, Danmark Overskrifter
Similar News:Du kan også læse nyheder, der ligner denne, som vi har indsamlet fra andre nyhedskilder.
Tom Bradby's Meghan Markle interview that 'cost him Prince William' friendshipITV journalist Tom Bradby found himself in the middle of brothers Prince William and Prince Harry after asking Meghan Markle if she was okay during a trip to southern Africa
Læs mere »
Prince Harry interview with Tom Bradby in fullPrince Harry opened up about his relationship with his family in a sit-down interview with ITV’s Tom Bradby that aired on Sunday, ahead of the release of his memoir Spare.
Læs mere »
12 jaw-dropping revelations from Harry's explosive Tom Bradby interviewPrince Harry's explosive interview with ITV News at Ten's Tom Bradby has finally aired, and the Duke of Sussex made several shocking claims about the Royal Family
Læs mere »
26 Of The Greatest Tom Ford For Gucci Red-Carpet LooksYes, there’s Gwyneth’s velvet suit, but Halle Berry’s bustier dress is well worth revisiting, as is Demi Moore doing denim on the red carpet...
Læs mere »
Armed police swoop on bus after reports of shooting as two passengers injured'It's terrifying'
Læs mere »